1What is the ART PRP™ PLUS?
The ART PRP™ PLUS concentration system has an integrated filter system for the concentration of proteins from the platelet poor plasma (PPP). By transferring the plasma from one syringe on Port “B” to another syringe on Port “C” the growth factors and beneficial proteins travel through hundreds of hollow, nanoporous fibers. During this process, water and salt ions are pushed out while the beneficial proteins are retained and concentrated.

The PPP can be passed multiple times from syringe to syringe through the filter until the desired volume is obtained. The PPP contains proteins like Alpha-2 Macroglobulin (A2M), Interleukin-1 Receptor Antagonist Protein (IRAP), Vascular Endothelial Growth Factor (VEGF), Platelet-derived Growth Factor (PDGF), Transforming Growth Factor Beta (TGF-Beta), Fibroblast Growth Factor (FGF), Fibronectin and Fibrinogen. 1,2,3

A2M is a powerful natural plasma protease inhibitor, cytokine carrier, and ligand for cell-signaling receptors, and is synthesized primarily in the liver but dispersed through the blood plasma in whole blood and bone morrow. 3,6 A2M stops the progress of Osteoarthritis (OA) by preventing degenerative cartilage breakdown and assisting with cartilage repair. 1,2,5 It can also inhibit inflammatory cytokines, and it thus disrupts inflammatory cascades.
In its plasma-soluble tetramer form, it encapsulates matrix metalloproteinases (MMPs) and other destructive enzymes common to arthritic tissue and slows or halts the breakdown of articular cartilage surfaces and other forms of extracellular matrix (ECM).7 A2M also binds pro-inflammatory molecules TNF- , TGF- , and IL-1. While IRAP is present at much higher levels in bone marrow aspirate, the amount available in the whole blood is still concentrated with the ART PRP Plus system.
  • Nearly closed systems offers multiple connection ports
  • Thumb-wheel allows precise recovery of the targeted cell layer
  • Large aperture collection window
  • Manual collection accommodates full spectrum of hematocrit levels
  • Adjustable flow valve diverts fluid without additional sterile breaks
  • Adjustable flow valve simplifies seperation/combining of cells and proteins
  • ART PRP™ Plus concentrates platelet poor plasma (PPP) using nanoporous fibers
  • ART PRP™ Plus reduces 60 mL whole blood to 25-30 mL of PPP and nano-filtration further concentrates to 3-5 mL concentrated PPP
  • Minimizes sterile breaks where contamination occurs
  • Easy seperation of fluid fractions (e.g. PRP, PPP); Processes anywhere from 50 cc up to 60 cc of whole blood
  • Eliminates turbulence reducing cell velocity that can lyse cells
  • Increases cell recovery rates; other fully automated designs compromise recovery rate
  • Keeps Buffy Coat in the collection zone yielding 3 cc to 5 cc on average of PRP (Buffy Coat) from a typical 60 cc volume of whole blood
  • Manual manipulation is the critical feature that produces optimal recovery and a customized final product
  • Provides for the highest concentration so that more cells can be delivered in the smaller volume